PCR

Atomic Absorption spectrophotometer


Working principles (With illustration)

In AAS, liquid sample is aspirated to become aerosols of fine particles (nebulization), Flame vaporizes the aerosols (atomization), Elevated temperatures in a flame or furnace changes the chemistry of atoms. Temperature affects the ratio of excited and unexcited atoms.Beer’s law is used to calculate concentration of specific element.

Application and studies related to

  • Concentration of metals in water, sediment and biotic community. In laboratory routinely analysed concentrations of Cd, Cu, Fe, Mn, Pb and Zn by flame mode. Arsenic by hydride generation mode.

    • User Instruction (how to bring samples)

  • Water samples to be preserved with adding conc. HNO 3 after filtration. Fresh biological samples may be ice preserved or dried. Then all the samples are digested with suitable acid to bring metals into free solution phase. All the samples to be passed through filtration (Whatman 42 or equivalent) before feeding into instrument. The processed samples are now ready for analysis in AAS.For analysis of arsenic in hydride generation mode, the acid for processing of water samples is HCl.

    • User name:
      Dr. S. Samanta
    • Instrument Details:
    • Make
      GBC, Australia
    • Model:
      Avanta Sigma
    • Specification

      Under repair
    • Current Status of Instrument:
      Under repair
    • Charges including GST
    • Name of Analysis Price per Sample (in ₹)) Remarks
      Metal Analysis(For each metal per sample: Cd, Cu, Fe, Mn, Pb, Zn and As) 1000  (Industry / Enterpreneur)
      500    (Research/Academic/Farmer)
      		 Nil
    • Contact details with e-mail & phone:
    • Director,
      ICAR- Central Inland Fisheries Research Institute
      Barrackpore, Kolkata-700120, W.B.
      Telefax: 033 25920177
      E- mail: director.cifri@gmail.com
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    This website belongs to ICAR-Central Inland Fisheries Research Institute, Indian Council of Agricultural Research, an autonomous organization under the Department of Agricultural Research and Education, Ministry of Agriculture & Farmers Welfare, Government of India. Copyright @ 2010 ICAR, This website is developed & maintained by Agricultural Knowledge Management Unit. 2017 Last updated on 17/12/2017
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